Otimização da produção de lipases em cultivo submerso utilizando leveduras do cerrado tocantinense

Abstract

The Cerrado is the second largest biome in South America, housing 11.627 native plant species already cataloged. The great industrial demand for new sources of lipases with different characteristics has stimulated the search for new strains of lipolytic microorganisms. The purpose of this study is to optimize the lipase production in submerged culture for little studied yeast. A Plackett-Burman design was used to evaluate the effect of ten variables (pH, agitation, ammonium sulfate, peptone, Triton X-100, Tween 20, olive oil, yeast extract, magnesium sulfate and potassium dihydrogenphosphate) in the lipase production by two yeast strains, considering a significance level of p < 0,1. The significant variable for strain TAQ 616 lipases production was the olive oil concentration and for biomass were olive oil, triton X-100 and KH2PO4. The enzymatic activity of lineage ABRT 461suffered the influence of pH, agitation and KH2PO4, and for biomass production any variable was statistically significant. The ABRT 461 lineage was selected to do a central composite rotational design (CCRD)2³. The optimum conditions for enzyme activity generated by the proposed model was 114 rpm, pH 5.0 and 25 g.L-1 concentration of (NH4)2SO4. The enzymatic activity of 84,4 U.mL-1 was obtained after the cultivation of ABRT 461 lineage for 72 hours in optimal conditions estimated by the model.