Estudo do perfil químico e bioatividade de frações do fungo endofítico Neopestalotiopsis sp. CG13

Abstract

In previous studies, the endophytic fungus Neopestalotiopsis sp. CG13, isolated from Clitoria guianensis (a plant found in the Cerrado of Tocantins), showed the ability to act as a biocontrol agent against phytopathogenic fungi, such as Sclerotinia sclerotiorum and Fusarium sp. The present study evaluated the chemical profile of the subfractions obtained from the cultivation of Neopestalotiopsis sp. CG13 on solid medium (rice), using liquid chromatography coupled to mass spectrometry and the online platform GNPS2 for compound annotation and construction of molecular networks. Using the molecular network obtained in GNPS2, 14 compounds were recorded, namely: phenolphthalein, cyclo(tyrosyl-prolyl), vitamin E acetate, glycerophosphocholine, octocrylene, prolifenylalanine, 4- oxododecanedioic acid, beauvericin, monopalmitin, ergosterol peroxide, N-acetyl-L-tyrosine, LPC (18:2), denopamine, cyclo(L- isoleucyl-L-prolyl) and asperfenamate. In addition to the chemical analysis, the cytotoxicity of the human subfractions against HaCaT (non-tumor) and HCT-116 (tumor) were evaluated. There was no specific cytotoxic activity, however, the Fr5 subfraction presented an initial selectivity value equal to 1.8, demonstrating some cytotoxicity and the presence of cytotoxic bioactive compounds. Regarding antibacterial activity, there was no considerable inhibition of the fractions in relation to the bacteria Stenotrophomonas maltophilia and Escherichia coli, however, the Fr5 fraction affected the formation of E. coli colonies.