Comparação da comunidade microbiana na câmara pulpar de dentes hígidos de cadáveres e pacientes para possível estimativa do tempo de morte

Abstract

The Post-Death Interval (PMI) is the period of time that has elapsed since the occurrence of death until the moment when the body and/or human remains are studied. The estimation of this range is important role subject in importance for your criminal resolution. Teeth are fundamental structures in this context, due to their high strength and specificity. Therefore, the objective is to evaluate the presence of microorganisms in the dental pulp after establishing parameters that can be killed, with a view to predicting studies in situations where it is necessary to determine the time of death if necessary. For this, microbiological tests were performed to detect and quantify bacteria and fungi in pulp tissues extracted from 46 teeth belonging to live (32) and live (14) animals. Results from 5 h of fungi were observed from IPMs for live teeth as well as 13 h PM teeth for live cadavers. Still, smaller IPM present greater contamination by bacteria and fungi, however, a predominance of fungus was greater in cadavers and bacteria in living. This method can become an important tool, and future work may include an identification of fungal and bacterial species, similar to what is done with forensic entomology.